Can I use CMB2’s show_on_cb within another Plugin instead of functions.php?

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I’m trying to setup some metaboxes outside the functions.php file along with some custom post types.

I have the cpts and now the cmb2 metaboxes working but I’m having trouble figuring out how to use a show_on_cb. I guess how I use the add_action and add_filter are throwing me.

If I have CMB2 installed as a Plugin and I’m making a Plugin, should include everything in an initialize just like a functions.php approach?

Thanks